Sheep B-cell lymphoma factor 2 (Bcl-2) ELISA Kit

The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100 Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.

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Description:

Name	Sheep B-cell lymphoma factor 2 (Bcl-2) ELISA Kit
Specifications	96T
article number	HB089-Sp
Species	Sheep
Sample type	serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc.
Detection range	See the manual for details
Sensitivity	See the manual for details
Response time	1-5h
Sample volume	10ul
Detection wavelength	450nm
Delivery date	3-5 days
Manual	Consult our salesperson for obtaining

Precision

The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100
Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.
Inter-batch difference: select 3 different batches of kits to quantitatively determine the low, medium, and high-value fixed value samples. Use the same kit for each sample to repeat the measurement 10 times, and calculate the average value and SD of samples with different concentrations. value6
Intra-run difference: CV<4.5%
Difference between batches: CV<7.5%

Recovery rate

The target protein of known concentration was added to 5 different samples, and the recovery experiment was performed to obtain the recovery range and average recovery rate.

Sample Type	Range (%)	Average Recovery (%)
Tissue Lysates (n=5)	93-109	97
Serum (n=5)	88-107	94
EDTA plasma (n=5)	85-106	93
Cell culture media (n=5)	92-105	95

Linear

Add the target protein of known concentration to 5 samples respectively, do recovery experiment, get the range of recovery rate and average recovery rate. Dilute 5 samples by 2 times, 4 times, 8 times, and 16 times respectively for recovery experiments to obtain the recovery range and average recovery rate.

Sample	1:2	1：4	1:8	1:16
Tissue Lysates (n=5)	92-105%	84-99%	90-102%	80-93%
Serum (n=5)	81-101%	89-99%	86-98%	83-101%
EDTA plasma (n=5)	85-97%	82-101%	83-96%	79-91%
Cell culture media (n=5)	83-105%	89-112%	81-106%	82-110%

Specificity

This kit recognizes natural and recombinant Sheep B-cell lymphoma factor 2 (Bcl-2) ，and has no obvious cross-reactivity with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross-reaction test of all related or similar substances. Therefore, this kit may have cross-reaction with other untested substances.

Stability

It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.

Kit composition

Serial number	English name	specification
1	Wash solution(30X)	20ml×1 bottle
2	HRP-Conjugate reagent	6ml×1 bottle
3	Microelisa stripplate	12 holes×8 strips
4	Sample diluent	6ml×1 bottle
5	Chromogen Solution A	6ml×1 bottle
6	Chromogen Solution B	6ml×1/bottle
7	Stop solution	6ml×1 bottle
8	Standard	0.5ml×1 bottle
9	Standard diluent	1.5ml×1 bottle
10	Product Description	1 serving
11	Sealing plate membrane	2 sheets
12	Sealing bag	1 piece

Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.
Materials and consumables needed but not provided

1. Microplate reader (it is recommended to warm up the instrument before use)
2. Precise pipettes, tips, and sample tanks
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37℃ water bath or thermostat
6. EP tube
7. Powder-free disposable latex gloves

Storage conditions and validity period
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.